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Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)
Reference : E-ISAMYFO
CAS Number : 9067-73-6
Enzyme Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Enzyme Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum)
Shipping temperature: ambient
Storage temperature: 4oC
Formulation: in 3,2 M ammonium sulphate
Physical form: suspension
EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6
Synonyms: isoamylase; glycogen 6-α-D-glucanohydrolase
Source: Flavobacterium odoratum
Highly purified
Molecular weight: 83,000
Concentration: supplied at ~ 200 U/mL
Specific activity:
~ 140 U/mg (40oC, pH 6.0 with 1 mM CaCl2 on oyster glycogen)
Unit definition:
One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40oC.
Specificity:
Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins
Temperature optima: 50°C
pH optima: 6
Method recognition: AOAC method 2000.11 and GB standard 5009.245-2016
Stability:> 4 years at 4°C
Shipping temperature: ambient
Storage temperature: 4oC
Formulation: in 3,2 M ammonium sulphate
Physical form: suspension
EC 3.2.1.68
CAZy Family: GH13
CAS: 9067-73-6
Synonyms: isoamylase; glycogen 6-α-D-glucanohydrolase
Source: Flavobacterium odoratum
Highly purified
Molecular weight: 83,000
Concentration: supplied at ~ 200 U/mL
Specific activity:
~ 140 U/mg (40oC, pH 6.0 with 1 mM CaCl2 on oyster glycogen)
Unit definition:
One Unit of isoamylase activity is defined as the amount of enzyme required to release one µmole of glucose reducing sugar equivalents per minute from oyster glycogen (10 mg/mL) in MES buffer (100 mM, pH 6.0) plus 1 mM CaCl2 at 40oC.
Specificity:
Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins
Temperature optima: 50°C
pH optima: 6
Method recognition: AOAC method 2000.11 and GB standard 5009.245-2016
Stability:> 4 years at 4°C
- High purity and stability
- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Carbohydrate research and in the food and feeds, and cereals industry
Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
- Carbohydrate research and in the food and feeds, and cereals industry
Pure Isoamylase (Glycogen 6-glucanohydrolase) (Flavobacterium odoratum) for use in biochemical enzyme assays and in vitro diagnostic analysis. Isoamylase, Fructanase (E-FRMXPD) and Amyloglucosidase (E-AMGDF) are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
Data Booklet
Safety data sheet
The attached documents are subject to change. To receive the latest updates, please contact us.
Specifications
| Reference | Packaging | |
|---|---|---|
| E-ISAMYFO | 200 unités (units) | |
| E-ISAMYFO | 800 unités (units) |