Polyphenol quantification kit by the Folin-Ciocalteu (FC) method is convenient and simple.
In addition, it requires only common equipment and has produced a large body of comparable data. Under proper conditions, the assay can also quantify monophenols and gives predictable reactions with the types of phenols found in nature. Because different phenols react to different degrees, expression of the results as a single number—such as milligrams per liter gallic acid equivalence—is necessarily arbitrary.

This assay kit has been used as a measure of total phenols in natural products. Its basic mechanism is an oxidation/reduction reaction. The original F-C assay, used the carbonate buffer for pH adjustment. Additionally, the end-point of the reaction after 120 min at room temperature, which makes difficult its implementation for routine analysis .

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