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FRAP, antioxydant capacity
Reference : KF01003
FRAP antioxidant capacity assay kit
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Belgium, French overseas departments and territories, French Polynesia, Luxembourg, Switzerland, France (Corsica)
FRAP assay kit is recommended for total antioxidant activity of single antioxidants in aqueous solution and added to plasma.
The assay described here measures the ferric reducing ability of plasma (FRAP). At low pH, when a ferric complex is reduced to the ferrous form (Fe2+), an intense blue color with an absorption maximum at 593 nm develops.
This reaction is non specific and any half-reaction which has a less-positive redox potential, under reaction conditions, than the Fe3+/Fe2+ complex half reaction will drive Fe3+ complex reduction. Acidic conditions favor reduction of the complex and, thereby, color development, showed that an antioxidant is present.
Fe3+-C+AOH → Fe2+-C(blue) (λmáx= 593 nm)
The assay described here measures the ferric reducing ability of plasma (FRAP). At low pH, when a ferric complex is reduced to the ferrous form (Fe2+), an intense blue color with an absorption maximum at 593 nm develops.
This reaction is non specific and any half-reaction which has a less-positive redox potential, under reaction conditions, than the Fe3+/Fe2+ complex half reaction will drive Fe3+ complex reduction. Acidic conditions favor reduction of the complex and, thereby, color development, showed that an antioxidant is present.
Fe3+-C+AOH → Fe2+-C(blue) (λmáx= 593 nm)
Recommended for total antioxidant activity of single antioxidants in aqueous solution and added to plasma.
Biological samples; single antioxidants in aqueous solution and added to plasma.
Specifications
| Reference | Packaging | |
|---|---|---|
| KF01003-100 | 100 tests (96 puits/wells format) | |
| KF01003-200 | 200 tests (96 puits/wells format) | |
| KF01003-400 | 400 tests (96 puits/wells format) |