FAST FRAP assay kit is a ready-to-use kit, easy and highly reproducible assay to test TAC on single antioxidants in aqueous solutions, added to plasma and on beverages with the outstanding feature of being faster than the FRAP classical assay.
 
Antioxidant capacity is an overall ability of organisms or food to catch free radicals and prevent their harmful effect. Antioxidative effect includes protection of cells and cellular structures against the harmful effect of free radicals, especially oxygen and nitrogen. Substances with antioxidative properties are called antioxidants. They are contained in food and food supplements, most commonly in fruits, vegetables, rice, wine, meat, eggs, and another foodstuff of plant and animal origin.

Antioxidative systems include antioxidative enzymes, that is, superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase, and non-enzymatic substrates, such as glutathione, uric acid, lipoic acid, bilirubin, coenzyme Q, vitamin C (L-ascorbic acid), vitamin A (retinol), vitamin E (tocopherol), flavonoids, carotenoids, theine compounds in green tea, and others. Some biomolecules are also considered biologically active and clinically significant antioxidants, for example, transferrin, ferritin, lactoferrin, ceruloplasmin, hemopexin, haptoglobin, and uric acid.

Total antioxidant capacity or TAC has been considered an overall parameter, which alterations have been linked to several conditions as infertility, obesity, cancer and neurodegenerative diseases.

Assay principle
This kit measures the antioxidant activity of compounds that are able to reduce the ferric complex. When the complex is at an acidic pH, in the presence of a suitable antioxidant solution, it is reduced, which shows a maximum of absorbance at 593 nm. This reaction is rapid and proportional to the antioxidant capacity of the sample.