The Phytase Assay Kit is a simple, quantitative method which can be used to measure phytase activity. Analysis is based on the hydrolysis of phytic acid by phytase and quantitative measurement of the phosphate released. Results are measured using a standard UV-VIS spectrophotometer and do not require the creation of a standard curve. The Phytase method can be used to measure phytase activity in cereal, fungal and bacterial phytases, and can also be used for the analysis of phytase in animal feed samples.

Shipping temperature: ambient

Storage temperature:
- Short term stability: 2-8^oC,
- Long term stability: see individual component labels

Assay format: spectrophotometer

Detection method: absorbance - 360

Signal response: increase

Linear range: 0.1 to 10 μg of phosphate per assay

Reaction time: ~ 30 min

Detection limit: 1.5 U/L

Temperature Range: 40°C

pH Range: 5.5

Stability: > 2 years under recommended storage conditions

• Easier and quicker test than competitors
• No need for expensive instrumentation, i.e. fluorimeter
• Low cost per assay
• All reagents stable for > 2 years after preparation
• Standard included
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing

Phytase activity in cereal, fungal, bacterial phytases, and in animal feed samples.

Safety data sheet Data calculator Data Booklet

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