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Lactose - sequential - high sensitivity - Enzymatic assay kit
Reference : K-LOLAC
Innovation ! Rapid, novel, sequential method for the measurement of free-glucose and lactose in conventional, low-lactose and lactose-free dairy products
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
This sequential assay format reduces the manual input required by an analyst when compared to traditional lactose assay formats and therefore improves both accuracy and efficiency. When used in combination with the Creep Calculator provided, the β-galactosidase employed in this kit allows for the selective measurement of lactose in the presence of galacto-oligosaccharides (GOS) which are commonly found in lactose-free dairy products.
This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase.
Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
British Patent Application No. 1710170.0.
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer
Detection method: absorbance - 340
Signal response: increase
Linear range: 1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)
Reaction time: ~ 10 min
Detection limit: 1,62 mg/l
Stability: > 2 years under recommended storage condition
Method recognition: AOAC Method 2020.08
This constitutes a significant improvement over existing commercially available lactose assay kits which typically overestimate lactose content in lactose-free samples due to the unselective hydrolysis of GOS by β-galactosidase.
Lastly, the sensitivity of the K-LOLAC assay kit has been doubled through the use of a cascade biochemical pathway, helping to significantly reduce the LOD and LOQ for the assay.
British Patent Application No. 1710170.0.
Shipping temperature: ambient
Storage temperature:
- Short term stability: 2-8oC,
- Long term stability: see individual component labels
Assay format: spectrophotometer
Detection method: absorbance - 340
Signal response: increase
Linear range: 1 to 50 µg of lactose (or 0.50 to 25 µg of D-glucose)
Reaction time: ~ 10 min
Detection limit: 1,62 mg/l
Stability: > 2 years under recommended storage condition
Method recognition: AOAC Method 2020.08
- World’s first sequential assay for lactose, i.e. improves accuracy and efficiency
- Contains a specific β-galactosidase for the selective measurement of lactose in dairy products
- Efficient pre-treatment step allows for accurate measurement of lactose in “low-lactose” and “lactose-free” dairy products
- Lower limit of detection (LOD) than any other commercially available enzymatic lactose detection method. LOD at 1,62 mg/L
- Very competitive price (cost per test)
Dairy products (milk, cream, whey powder, cheese, sweetened condensed milk ...), foods containing milk (dietary products, bakery, chocolate, confectionery), food additives, animal food, cosmetics, pharmaceuticals, samples, organic farming and infant feeding,..
Safety data sheet
Data calculator
Validation report
K-LOLAC_Single_Lab_Validation
Flyer K-LOLAC
Data Booklet
The attached documents are subject to change. To receive the latest updates, please contact us.
Specifications
| Reference | Packaging | |
|---|---|---|
| K-LOLAC | 65 tests, adaptable en microplaque (suitable to microplate) |