Novel method for the specific, convenient, cost effective and rapid measurement of L-glutamine and ammonia in culture media/supernatants and other materials

Shipping temperature: ambient

Storage temperature:
- Short term stability: 2-8^oC,
- Long term stability: see individual component labels

Assay format: spectrophotometer, microplate

Detection method: absorbance - 340

Signal response: increase

Linear range: 1 to 40 µg of L-glutamine per assay

Reaction time: ~ 10 min

Detection limit: 0,54 mg/l (L-glutamine) / 0,06 mg/l (ammonia)

Stability: > 2 years under recommended storage condition

Novel method

• Very rapid reaction due to use of high activity glutaminase and uninhibited glutamate dehydrogenase
• All enzymes supplied as stabilised suspensions
• Only enzymatic kit available
• Very cost effective
• All reagents stable for > 2 years after preparation
• Spreadsheet available
• Standard included

Cell culture media and cultures, dietary supplements, vegetables and other materials (e.g. biological samples, etc.)

Safety data sheet Data calculator Data Booklet

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