Fructan HK (hexokinase format) - Enzymatic assay kit

Reference : K-FRUCHK

Method for the specific measurement of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
Method for the specific measurement of all fructo-oligosaccharides (reducing and non-reducing) and of fructan polysaccharides but is not suitable for the analysis of samples containing high levels of D-glucose, D-fructose, sucrose or maltose

Shipping temperature
ambient

Storage temperature
:

Short term stability: 2-8°C,
Long term stability: See individual component labels

Assay formatspectrophotometer

Detection methodabsorbance - 340

Signal responseincrease

Linear range4 to 80 µg of D-glucose, D-fructose or sucrose per assay

Total assay time: ~ 30 min

Detection limit: 1 g/100 g

Stability: > 2 years under recommended storage condition

Method recognition: this method is a modification of AOAC Method 999.03 and AACC Method 32-32.01

Note that this is a modified version of AOAC method 999.03/AACC Method 32.32.01 (K-FRUC) in which the overall assay principle remains unchanged but detection is performed through the enzymatic hexokinase/glucose-6-phosphate dehydrogenase system as opposed to the reducing sugar PAHBAH determination. The K-FRUCHK procedure has not received official method status.

 
  • All reagents stable for > 12 months after preparation
  • Fructan kits are available only from Megazyme
  • Simple format
  • Spreadsheet available
  • Standard included
  • Very cost effective
Flours, plant materials (e.g. onion), food products and other materials
Data Booklet Safety data sheet Calculator data Performance product


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Specifications

Reference Packaging  
K-FRUCHK 50 tests