The Digestible and Resistant Starch Assay Kit method is based on the research of Englyst et al. (Ref) with some modifications.

In line with Englyst definitions:

- Rapidly digestible starch (RDS) is that starch which is digested within 20 min.
- Slowly digestible starch (SDS) is that starch which is digested between 20 and 120 min.
- A new term, ‘Total digestible starch (TDS)’ is introduced (and measured) to cover all starch that is digested within 4 h (the average time of residence of food in the human small intestine).
- Resistant starch (RS) then, is that starch which is not digested within 4 h.
 

Shipping temperature: ambient

Storage temperature:
- Short term stability: 2-8^oC,
- Long term stability: see individual component labels

Assay format: spectrophotometer, microplate

Detection method: absorbance - 510

Signal response: increase

Linear range: 5 to 100 µg of D-glucose per assay

Total assay time: ~ 360 min

Detection limit: 2 g/100 g

Stability: > 2 years under recommended storage conditions

• The incubation conditions parallel those used in AOAC Method 2017.16, a new, rapid integrated procedure for the measurement of total dietary fiber (K-RINTDF).
• This method is physiologically based and designed to fit the definition of DF announced by Codex Alimentarius in 2009
• Digestion is performed using saturating levels of pancreatic α-amylase (PAA) and amyloglucosidase (AMG), but in stirred containers rather than shaken tubes, to simplify sample removal

Plant materials, starch samples and other materials

Data Booklet Safety data sheet Calculator data

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