Enzyme Oligo-1,6-α-Glucosidase plus β-Galactosidase

Content: 10,000 Units Oligo-α-1,6-Glucosidase plus 10,000 Units β-galactosidase

Shipping temperature: ambient

Storage temperature: 4^oC

Formulation: in 3,2 M ammonium sulphate

Physical form: suspension

EC: oligo-α-1,6-glucosidase: 3.2.1.10 / β-galactosidase: 3.2.1.23
CAZy Family: GH13 / GH35
CAS: oligo-α-1,6-glucosidase: 9032-15-9 / β-galactosidase: 9013-11-2 

Synonyms: oligo-α-1,6-glucosidase: oligosaccharide 6-alpha-glucohydrolase / β-galactosidase: beta-D-galactoside galactohydrolase

Source: oligo-α-1,6-glucosidase: Microbial / β-galactosidase: Aspergillus niger

Molecular weight: oligo-α-1,6-glucosidase: 67,300 / β-galactosidase: 125,000

Concentration: oligo-α-1,6-glucosidase (2,000 U/mL) / β-galactosidase (2,000 U/mL)

Specific activity:
Oligo-α-1,6-glucosidase (~ 170 U/mg) / β-galactosidase (~ 170 U/mg)

Unit definition:
Oligo-α-1,6-glucosidase: One Unit of oligo-α-1,6-glucosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-D-glucopyranoside (5 mM) in sodium acetate buffer (50 mM), pH 4.5 at 40°C.

β-galactosidase: One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-β-D-galactoside (10 mM) in sodium acetate buffer (100 mM), pH 4.5 at 40°C.

Specificity:
This enzyme mixture gives complete hydrolysis of isomaltose and lactose under the defined assay conditions, with no hydrolysis of the trisaccharide, β-D-Fruf-(2,1)-β-D-Fruf-(2,1)-β-D-Fruf. 

Temperature optima: 50°C
pH optima: 4,5

Stability: > 3 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis

Data Booklet Safety data sheet

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