Fructanase - Mixture (ultrapure, recombinant, liquid)

EC number:
- Endo-inulinase: 3.2.1.7
- Exo-Inulinase: 3.2.1.80

CAZy family: GH32
CAS number: 9001-57-4, 9025-67-6, 37288-56-5

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 50% (v/v) glycerol + 0,02% sodium azide

Physical form: solution

Synonyms:
- Endo-inulinase: 1-β-D-fructan fructanohydrolase
- Exo-inulinase: fructan β-fructosidase

Source: Aspergillus niger
Recombinant from Aspergillus niger

Concentration:
- Endo-inulinase: supplied at ~ 1,000 U
- Exo-inulinase: supplied at ~ 10,000 U

Specificity:
- Endo-inulinase: endo-acting hydrolysis of (2,1)-β-D-fructosidic linkages in inulin
- Exo-inulinase: hydrolysis of terminal, non-reducing β-D-fructofuranoside residues in β-D-fructofuranosides

Unit definition:
- Endo-Inulinase: one unit of endo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from inulin (20 mg/mL) in sodium acetate buffer (100 mM), pH 4,5
- Exo-Inulinase: one unit of exo-inulinase activity is defined as the amount of enzyme required to release one μmole of β-D-fructose reducing-sugar equivalents per minute from kestose (5 mg/mL) in sodium acetate buffer (100 mM), at pH 4,5 at 40°C

Temperature optima: 60°C

pH optima: 4,5

Method recognition: AOAC Method 997.08 and AOAC Method 2000.11

Stability: Minimum 1 year at < -10°C

• High purity and stability

Research, biochemical enzyme assays and in vitro diagnostic analysis

Data booklet Safety data sheet

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