Enzyme Cellobiohydrolase I

Shipping temperature: ambient

Storage temperature: 2-8^oC

Formulation: in 3,2 M ammonium sulphate
Electrophoretically homogeneous (MW 65,000)

Physical form: suspension

EC 3.2.1.176
CAZy Family: GH7
CAS: 37329-65-0

Synonyms: cellulose 1,4-β-cellobiosidase (non-reducing end); 4-β-D-glucan cellobiohydrolase (non-reducing end)

Source: Trichoderma longibrachiatum
Highly purified

Molecular weight: 65,000

Concentration: supplied at ~ 0,1 U/mg

Specific activity:
~ 0,1 U/mg (40°C, pH 4,5 on CM-cellulose 4M). Active on p-nitrophenyl-β-lactoside

Unit definition:
One Unit of cellobiohydrolase I activity is defined as the amount of enzyme required to release one micromole of glucose-reducing-sugar equivalents per minute from CM-cellulose 4M (10 mg/mL) at pH 4,5 and 40°C

Also active on pNP β-lactoside and pNP β-cellobioside where one Unit of cellobiohydrolase I activity is defined as the amount of enzyme required to release one micromole of p-nitrophenol from these substrates (2 mM) at pH 4,5 and 40°C

Specificity:
Hydrolysis of (1,4)-β-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains. Also active on pNP β-lactoside and pNP β-cellobioside

Temperature optima: 60°C
pH optima: 5,5

Stability: > 4 years at 4°C

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis
- Diagnostics and research within the textiles, food and feed, carbohydrate and biofuels industries

Data Booklet Safety data sheet

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