Enzyme Amyloglucosidase

Shipping temperature: ambient

Storage temperature: 2-8°C

Formulation: in buffered solution

Physical form: solution

EC 3.2.1.3
CAZy Family: GH15
CAS: 9032-08-0

Synonyms: glucan 1,4-α-glucosidase; 4-αa-D-glucan glucohydrolase;glucoamylase

Source: Aspergillus niger
Recombinant from Aspergillus niger

Molecular weight: 143,500

Specific activity:
~ 36 U/mg (40°C, pH 4,5 on soluble starch)
~ 400 U/mL on p-nitrophenyl Beta-maltoside
~ 6,520 U/mL on soluble starch

Unit definition:
One Unit of amyloglucosidase activity is defined as the amount of enzyme required to release one µmole of glucose reducing-sugar equivalents per minute from soluble starch (10 mg/mL) at pH 4,5 at 40°C

Specificity:
Hydrolysis of terminal (1,4)-linked α-D-glucose residues successively from non-reducing ends of the chains with release of β-D-glucose

Temperature optima: 70°C
pH optima: 4

Stability: > 4 years 4°C

The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).

• High purity and stability

- Research, biochemical enzyme assays and in vitro diagnostic analysis.
- This enzyme is recommended for use in Total Dietary Fibre analytical procedures and the Total Starch test method.
The preparation is free of glycerol so can be used in TDF procedures where glycerol is used as an internal standard (e.g. AOAC Method 2001.03/AACC Method 32-41.01; the Matsutani Method).

Data Booklet 20 ML Data Booklet 50 ML Safety data sheet

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