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α-L-Arabinofuranosidase (Aspergillus niger)
Reference : E-AFASE
CAS Number : 9067-74-7
Enzyme α-L-Arabinofuranosidase
Countries eligible for delivery
Countries eligible for delivery:
France (except Corsica and overseas departments and territories), Algeria, French overseas departments and territories, French Polynesia, Libyan Arab Jamahiriya, Morocco, Tunisia, Egypt, France (Corsica)
EC 3.2.1.55
CAZy Family: GH51
CAS: 9067-74-7
Synonyms: non-reducing end α-L-arabinofuranosidase;α-L-arabinofuranoside non-reducing end α-L-arabinofuranosidase
From Aspergillus niger.
In 3,2 M ammonium sulphate.
Supplied at ~ 300 U/mL.
Specific activity:
~ 32 U/mg (40°C, pH 5.5 on p-nitrophenyl-alpha-L-arabinofuranoside);
~ 12.6 U/mg (on sugar-beet arabinan);
~ 1.2 U/mg (on wheat arabinoxylan).
Stability: > 4 years at 4°C.
Unit definition:
One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4,0 at 40°C.
Specificity:
Hydrolysis of α-1,2- and alpha-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses alpha-1,5-linked arabino-oligosaccharides at a much lower rate.
Temperature Optima: 40°C
pH Optima: 4
CAZy Family: GH51
CAS: 9067-74-7
Synonyms: non-reducing end α-L-arabinofuranosidase;α-L-arabinofuranoside non-reducing end α-L-arabinofuranosidase
From Aspergillus niger.
In 3,2 M ammonium sulphate.
Supplied at ~ 300 U/mL.
Specific activity:
~ 32 U/mg (40°C, pH 5.5 on p-nitrophenyl-alpha-L-arabinofuranoside);
~ 12.6 U/mg (on sugar-beet arabinan);
~ 1.2 U/mg (on wheat arabinoxylan).
Stability: > 4 years at 4°C.
Unit definition:
One Unit of α-L-arabinofuranosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol (pNP) per minute from p-nitrophenyl-α-L-arabinofuranoside (5 mM) in sodium acetate buffer (100 mM), pH 4,0 at 40°C.
Specificity:
Hydrolysis of α-1,2- and alpha-1,3-linked L-arabinofuranose residues from arabinoxylans and branched arabinans. Hydrolyses alpha-1,5-linked arabino-oligosaccharides at a much lower rate.
Temperature Optima: 40°C
pH Optima: 4
- High purity and stability
- Research, biochemical enzyme assays and in vitro diagnostic analysis.
- Carbohydrate and biofuels research
- Carbohydrate and biofuels research
Data booklet
Safety data sheet
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Specifications
| Reference | Packaging | |
|---|---|---|
| E-AFASE | 480 unités (units on pNP-α-Arabf at 40°C) |